About / Lab Staff
Darina Lyudmilova Kachakova
Education:
03.2010~ PhD student, Department of Medical Chemistry and Biochemistry, Medical University, Sofia
2009 Master of science- Sofia University Kliment Ohridski, Faculty of Biology
2007 Bachelor Molecular biology- Sofia University Kliment Ohridski, Faculty of Biology
Previous and Current Employment:
06.2009- 04.2010 Lab technician, Molecular Medicine center
03.2009- 04.2009 Medical technician, National genetic laboratory
Language proficiency:
English
Research interests:
Oncogenetics, epigenetics, pharmacogenetics, genetics of multifactorial diseases, gene expression in cancer, DNA diagnostics
Participation in research projects:
Principal Investigator
2011-2012 Association study of polymorphic variants in chromosome locus 11p15 and evaluation of their significance for development of prostate cancer
2012-2013 Expression profiling of miRNAs in prostatic tissues and plasma of Bulgarian patients
Co-Investigator
2009-2012 National University Complex in Biomedical and Translational Research, infrastructural project
2009-2011 Clinical and molecular markers with predictive value for survival, quality of life and treatment response in high-grade gliomas
2009-2012 Investigation of genetic markers linked with increased risk and searching for diagnostic, prognostic and expression markers of prostate cancer
2010-2011 Analysis of promoter hypermethylation of biomarkers in urine of patients with prostate cancer - validation of their diagnostic value in Bulgarian population
2009-2010 Molecular diagnostics of deafness
2010-2012 Follow up of circulating tumor cells (CTC) levels in patients with metastatic breast carcinoma - prognostic and predictive meaning
2010-2011 Enhancing the capacity of the cores Cell and Tissue Cultures, Microarray and Laser Microdissection in Molecular Medicine Center, MU - Sofia, infrastructural project
2010-2012 Analysis of clinical and molecular characteristics of laryngeal squamous cell carcinoma and their application for prediction of prognosis and treatment response
2012-2013 Levels of expression of miRNAs in blood and synovial liquid as potential biomarkers for diagnosis, determination of severity and prognosis of rheumatoid arthritis
2012-2013 Pilot study of the gene RELN role in the pathogenesis of otosclerosis
2012-2013 Genetic aspects of nonsyndromic hearing loss- connexins 26 and 30
Publications:
1. Eeles RA, Kote-Jarai1Z, Olama AA,.Kaneva R, Slavov C, Mitkova A, Kachakova D…, The UK Genetic Prostate Cancer Study Collaborators/British Association of Urological Surgeons Section of Oncology, The UK ProtecT Study Collaborators, The PRACTICAL Consortium, Easton DF, Identification of seven novel prostate cancer susceptibility loci through a genome-wide association study, Nature Genetics 2009, Oct;41(10):1116-21. Epub 2009 Sep 20.
2. Kote-Jarai, Sof?a, Ali Amin Al Olama, Graham G. Danielle M. Karyadi ...... Douglas F Easton, Rosalind A Eeles, …(Darina Kachakova). Seven prostate cancer susceptibility loci identified by a multi-stage genome wide association study. Nature Genetics,43, (8), 2011 August, 785-791
Presentations:
1. Kaneva Radka, Kachakova Darina, Mitkova Atanaska, Popov Elenko, Vlahova Aleksandrina, Dikov Tihomir, Christova Svetlana, Kremensky Ivo, Mitev Vanio, and Slavov Chavdar, CYP1B1 polymorphic variants associated with prostate cancer risk in Bulgaria, Poster presentation. European Human Genetics Conference, 23 – 26 May 2009 Vienna, Austria.
2. Kachakova Darina, Mitkova Atanaska, Popov Elenko, Vlahova Aleksandrina, Dikov Tihomir, Christova Svetlana, Kremensky Ivo, Mitev Vanio, Kaneva Radka, and Slavov Chavdar, Association of SRD5A2 polymorphic variants with prostate cancer risk in Bulgaria, Poster presentation. 8th Balkan Meeting on Human Genetics, 14 – 17 May 2009 Cavtat, Croatia
3. Kachakova D, Popov E, Mitkova A, Dodova R, Vlahova A, Dikov T, Christova S, Slavov C, Mitev V and Kaneva R, Association study of polymorphic variants in chromosome locus 8q24 linked with prostate cancer in Bulgarian patients, 26-29 June 2010, Oslo, European Association of Cancer Research
4. Kachakova D, Popov E, Mitkova A, Dodova R, Vlahova A, Dikov T, Christova S, Slavov C, Mitev V and Kaneva R, Polymorphic variants in CYP1B1 linked with increased risk for prostate cancer in Bulgarian patients, The European Human Genetics Conference, 12-15 June 2010, Gothenburg, Sweden
5. Bichev S, Hristova S, Slavova Y, Vajarova R, Kachakova D, Kaneva R, Kremensky I, Implementation of qPCR and sequencing for KRAS and EGFR mutation detection in Bulgarian patients with colorectal and lung cancer, 26-29 June 2010, Oslo, European Association of Cancer Research
6. Popov E, Kachakova D, Boyadjieva M, Mitkova A, Dodova R, Vlahova A, Dikov T, Christova S, Kremensky I, Slavov Ch, Mitev V, Kaneva R, Role of genetic polymorphism in SRD5A2 gene in progression of prostate cancer, IX International Congress of Medical Sciences, Sofia, Bulgaria, 13-16 may, 2010
7. Popov E, Kachakova D, Mitkova A, Kaneva R, Vlahova A, Dikov T, Slavov C, Mitev V, Kremensky I, Prostate cancer risk association with SNP polymorphic variants of CYP1B1 gene in the Bulgarian population, 25th Anniversary EAU (European Association of Urology) Congress, Barcelona, Spain, 16-20 April 2010
8. Kachakova D, Popov E, Mitkova A, Dodova R, Vlahova A, Dikov T , Christova S, Slavov C, Mitev V and Kaneva R, “Association analysis in Bulgarian prostate cancer patients adds support to a newly identified susceptibility locus on chromosome 8q24”, The European Human Genetics Conference, 28-31 May 2011, Amsterdam, The Netherlands, /vol. 19, supplement 2, P06.181, p. 231
9. Vlahova A, Dikov T , Christova S, Kachakova D, Mitkova A, Genadieva M, Popov E , Slavov C, Mitev V and Kaneva R, “AMACR polymorphic variant rs3195676 and prostate cancer aggressiveness in Bulgarian patients”, 23rd European Congress of Pathology, 27 August- 1 September 2011, Helsinki, Finland, Virhows archive, The European Journal of Pathology, Vol 459,Supplement 1, p. S294
10. Kachakova D, Popov E, Mitkova A, Beltcheva O, Vlahova A, Dikov T, Christova S, Slavov C, Mitev V, Kaneva R, “Promoter hypermethylation of HIST1H4K and RASSF2 in urine from prostate cancer patients”, European Association for Cancer Research conference, 7-10 July 2012, Barcelona, Spain, /vol.48, supplement 5, 520, p. S123-124
11. Marinova D, Stancheva G, Kachakova D, Mitkova A, Slavova Y, Maksimov V, Kaneva R, Mitev V, “Gene expression of EGFR and Mina53 in different histological subtypes of lung cancer”, European Association for Cancer Research conference, 7-10 July 2012, Barcelona, Spain, /vol.48, supplement 5, 627, p. S148-149
12. Marinova D, Kachakova D, Stancheva G, Slavova-Marinova Y, Maksimov V, Mitkova A, Kaneva R, Mitev V, “Gene expression analysis of MEN1 and EGFR in operated for primary lung cancer patients”, 24th European Congress of Pathology, 8-12 September 2012, Prague, Czech Republic, Virhows archive, The European Journal of Pathology, Vol 461, Supplement 1, p. S169
